纤溶酶和透明质酸酶在诱导猪玻璃体后脱离中对眼前部组织的安全性研究(英文)

Study on the safety of eye anterior tissues during plasmin and hyaluronidase inducing posterior vitreous detachment in pigs

目的:研究纤溶酶和透明质酸酶玻璃体内注射诱导猪玻璃体后脱离对眼前部组织的影响,探讨其眼前部组织的安全性。方法:15只健康无眼疾贵州小型香猪,分为A,B,C组,每组5只,每只1眼为实验眼,另1眼为对照眼,实验组与对照组各15眼。实验眼玻璃体内注射酶,A组50U(0.1mL)透明质酸酶,B组0.5U(0.1mL)纤溶酶,C组0.5U(0.05mL)纤溶酶+50U(0.05mL)透明质酸酶,对照眼注射等量BSS液。注射前后分别行裂隙灯显微镜、间接眼底镜、Sch!tz眼压计临床检查。7d后摘除眼球,行组织学检查。眼前部组织角膜、虹膜做光镜观察,睫状体、晶状体上皮做透射电镜超微结构的研究。结果:7d后A,B,C组实验眼及各组对照眼无明显眼内炎症反应,术前术后眼压变化差异无统计学意义。实验眼光镜观察角膜、虹膜组织结构无明显异常,电镜观察晶状体、睫状体上皮,细胞形态规则,结构清晰,胞膜完整,周界清楚,连接紧密,对照眼光镜及电镜观察组织结构与实验眼无差异。结论:0.5U纤溶酶和50U透明质酸酶猪玻璃体内注射诱导玻璃体后脱离中安全,无眼前部组织的毒性病理变化。

AIM： To evaluate the safety of eye anterior tissue when plasmin （Pm） and hyaluronidase （HS） are injected into pigs＇ vitreouses to induce posterior vitreous detachment（PVD）. METHODS： 15 pigs without ocular diseases were randomly assigned to groups A,B,C （5 in each group）. For each pig, one eye was experimental, the other eye was control. The experimental eye received intravitreal injection with enzyme： group A： 50U（0.1mL） HS; group B： 0.5U（0.1mL） Pro; group C： 0.5U（0.05mL） Pm combined with 50U（0.05mL） HS; while the control eye received intravitreal injection with equivalent dose of balanced salt solution （BSS）. Postoperative reactions in the eyes were carefully observed by clinical examinations such as slit-lamp microscopy and direct and indirect ophthalmoscopy and measurement of intraocular pressure （IOP）. Eyeballs were extirpated 7 days after the operation and histological examination was carried out. The corneas and irises were observed under light microscope （LM）. The epithelia of ciliary bodies and lens were studied with transmission electron microscope （TEM）. RESULTS： In all experimental eyes and control eyes of group A, B and C, histological examination showed： under LM ,the corneas and irises of experimental eyes had no histological abnormality in structure; Under TEM, epithelial cells of dliary bodies and the lens were tightly arrayed with clear cellular boundaries, clear intracellular structure, intact cell membranes, and intact nuclei; There was no significant difference in the clinical examinations and in the change of IOP between preoperation and postoperation, between the experimental group and the control group. CONCLUSION： With 0.5U Pm and HS 50U injected into pigs＇ vitreouses to induce PVD, alone or combined with two enzymes, evaluation revealed no evidence of toxicity on the eye anterior tissue. The dosage was proved to be safe for eye anterior tissue.