环孢霉素A对球后成纤维细胞体外增殖的影响

Proliferative effects of cyclosporin A on cultured human retroocular fibroblast growth

目的:研究环孢霉素A(CsA)对体外培养的球后成纤维细胞增殖的影响。方法:用MTT法测定CsA对体外培养的正常人和甲状腺相关眼病(TAO)患者球后成纤维细胞增殖的影响。结果:经1,10,100,500,1000μg/LCsA作用48h后,正常人球后成纤维细胞的A值分别为0.253±0.018,0.210±0.016,0.173±0.032,0.134±0.013,0.119±0.022与对照组0.278±0.019相比,差异有统计学意义(P<0.05);经1,10,100,500,1000μg/LCsA作用48h后,TAO患者球后成纤维细胞的A值分别为0.221±0.025,0.191±0.036,0.150±0.017,0.118±0.039,0.110±0.011与对照组0.249±0.028相比,差异有统计学意义(P<0.05);随着浓度的增加,抑制作用增强。结论:CsA呈浓度依赖性抑制正常人和TAO患者球后成纤维细胞增殖。

AIM： To investigate the proliferative effects of cyclosporin A （CsA） on cultured human retroocular fibroblast growth. METHODS： Effects of CsA on cultured human retroocular flbroblast growth were measured with mythyl thiazolyl tetrazolium （MTT） assay in normal people and patients with thyroid associated ophthalmopathy （TAO）. RESULTS： The A values of 1, 10, 100, 500 and 1 000μg/L CsA were 0.253±0.018, 0.210±0.016, 0.173±0.032, 0.134± 0.013 and 0.119±0.022 after 48 hours in fibroblasts of normal subjects, with significant difference compared with that of the control （0.278±0.019, P〈0.05）. The A values of 1, 10, 100, 500 and 1 000μg/L CsA were 0.221±0.025, 0.191± 0.036, 0.150±0.017, 0.118±0.039 and 0.110±0.011 after 48 hours in retroocular fibroblast of TAO patients, with significant difference compared with that of the control （0.249± 0.028, P〈0.05）. The inhibitive effect of CsA grew with the increase of its concentration. CONCLUSION： The CsA has dose-dependent inhibitive effects on the fibroblests proliferation of both normal people and TAO patients.