银杏叶提取物对U937泡沫细胞IL-1β、TNF-α及IL-10表达的影响(英文)

Effects of Ginkgo biloba extract on expressions of IL-1β,TNF-α,and IL-10 in U937 foam cells

本研究考察了氧化低密度脂蛋白(ox-LDL)刺激的U937细胞致炎细胞因子白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、抗炎细胞因子白细胞介素10(IL-10)及其受体(IL-10R)的蛋白及mRNA的表达,同时观察银杏叶提取物(GbE)对它们的作用。U937细胞用100 mg.L-1ox-LDL刺激24 h形成泡沫细胞,同时分别加入不同浓度的GbE(0.1,1及10μg.L-1)共孵育,采用酶联免疫吸附试验(ELISA)及逆转录聚合酶链式反应(RT-PCR)方法检测IL-1β、TNFα-、IL-10和IL-10R的蛋白或mRNA表达。U937泡沫细胞组IL-1β、TNF-α、IL-10和IL-10R的蛋白或mRNA的表达较对照组显著增加(P<0.01)。GbE组IL-1β及TNF-α的蛋白和mRNA的表达水平明显降低,IL-10的蛋白、IL-10和IL-10R的mRNA表达水平明显提高,与U937泡沫细胞组相比差异显著(P<0.05,P<0.01)。GbE对U937泡沫细胞致炎细胞因子IL-1β及TNF-α表达的显著抑制作用,对抗炎细胞因子IL-10及其受体IL-10R表达的显著上调作用可能是其抗atherosclerosis(AS)的机制之一。

This study is to investigate the protein and mRNA expressions of pro-inflammatory and anti-inflammatory cytokines in U937 foam cells and effects of Ginkgo biloba extract （GbE） on the cytokines. U937 cells were cultured with different concentrations of GbE （0. 1, 1, and 10μg·L^-1） , and stimulated by 100 mg · L^-1 oxidized low density lipoprotein （ox-LDL） for 24 h. The expressions of interleukin-1β （IL-1β） , tumor necrosis factor-α （TNF-α） and interleukin-10 （IL-10） in culture solution were detected by enzyme-linked immunosorbant assay （ELISA） and reverse transcriptase polymerase chain reaction （RT-PCR）. The results showed that incubated with 100 mg·L^-1 ox-LDL for 24 h, the U937 cells became foam cells, the protein or mRNA expressions of IL-1β, TNF-α, IL-10, and its receptor IL-10R in U937 foam cells were higher markedly than those in normal U937 cells. When the cells were pretreated with GbE （0. 1, 1, and 10μg· L^-1 ）, the increases of IL-1β and TNF-α in U937 foam cells were remarkably inhibited, but IL-10 expression increased greatly. Especially when cells were pretreated with 10μg · L^-1 GbE, the protein and mRNA expressions of IL-1β and TNF-α were markedly lower than those in U937 foam cells. The protein expression of IL-10 and mRNA expressions of IL-10 and its receptor IL-10R were markedly higher than those in U937 foam cells. GbE inhibited production of pro-inflammatory cytokines IL-1β and TNF-α, but up-regulated the production of anti-inflammatory cytokine IL-10 and its receptor IL-10R in U937 foam cells, which might be related with its anti-atherosclerotic actions.