蛋白激酶C亚型在人胃癌组织中的表达及其意义

EXPRESSION AND SIGNIFICANCE OF PROTEIN KINASE C ISOFORMS IN HUMAN GASTRIC CARCINOMA

目的研究PKC亚型α、β1、β2及ε在人胃癌组织的表达,并探讨PKC亚型表达与胃癌组织分化程度、临床分期以及与淋巴结转移的关系。方法采用免疫组化方法观察胃癌组织及其癌旁组织中PKC亚型α、β1、β2及ε的表达。结果PKCα在46例胃癌组织中,80.3%呈阳性表达,其中21.74%呈强阳性表达,与癌旁组织相比,二者差异显著(χ2=4.813,P<0.05);PKCβ1、β2则呈阴性;20例癌旁组织中,PKCε70%呈阳性表达,均为(+),46例胃癌组织中84.8%呈阳性表达,其中26.1%呈强阳性,两者阳性率无显著差异(χ2=1.93,P>0.05),但染色强度差异显著(χ2=5.07,P<0.05)。结论1.胃癌组织和癌旁组织均表达PKCα和,εPKCβ1、β2则呈阴性。2.胃癌组织PKC亚型α、ε的表达较癌旁组织显著增强。3.PKCα、ε的表达与胃癌组织的分化程度、临床分期及有无淋巴结转移均无明显相关性。

Objective To investigate the expression of protein kinase C isoforms （alpha, beta 1, beta 2 and epsilon）, in human gastric carcinoma tissue and to discuss the correlation of cheir expression with the differentiation, clinical stage and lymph node metastasis of gastric carcimoma. Methods Immumohistochemical analysis was used. Results PKC alpha was positively expressed in 80.3% of 46 gastric cancer specimens, and strongly positive in 21.74%. Compared with paracancerous gastric tissues, the difference was remarkable （χ^2 = 4. 813, P〈0.05）. PKC beta 1 and beta 2 were negative. PKC epsilon was positively expressed in both paracancerous and cancerous tissues, but the staining was stronger in gastric carcinomas than in paracancerous gastric tissues. Conclusion 1. PKC alpha and epsilon are expressed in both cancerous and paracancerous gastric tissues, but PKC beta 1 and beta 2 are negative. 2. PKC alpha and epsilon are expressed stronger in gastric carcinoma than in paracancerous gastric tissue. 3. the expressions of PKC isoforms alpha and epsilon are not correlative with the differentiation, clinical stage and lymph node metastasis of gastric carcinoma.