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靶向IGFIR的siRNA抑制人肝癌裸鼠移植瘤的实验研究 被引量：3

PSUPER-siRNA-IGFIR inhibition of human liver cancer xenograft in nude mice
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摘要 目的探讨人胰岛素样生长因子1类受体(IGFIR)干扰质粒(PSUPER-siRNA-IGFIR)对人肝癌细胞株SMMC7721裸鼠移植瘤生长的抑制作用。方法建立人肝癌细胞株SMMC7721裸鼠移植瘤模型,将PSUPER-siRNA-IGFIR质粒转染入移植瘤中,观察对肿瘤生长的作用。结果PSUPER-siRNA-IGFIR对人肝癌细胞株SMMC7721裸鼠移植瘤有生长抑制作用。与对照组相比IGFIR和MVD的表达明显下降。结论PSUPER-siRNA-IGFIR能抑制肝癌裸鼠移植瘤的生长。 Objective To investigate the effect of PSUPER-siRNA-IGFIR plasmid in inhibiting the growth of human liver cancer cell SMMC7721. Methods Cancer xenograft was established by transplanting SMMC7721 cell to nude mice, and the PSUPER-siRNA-IGFIR plasmid was constructed and the graft cancer was treated with PSUPER-siRNA-IGFIR plasmid. The effect on tumor growth was monitored. Results PSUPER-siRNA-IGFIR plasmid can inhibit the growth of human liver cancer cell SMMC7721. The expression of IGFIR and MVD in human liver cancer cell SMMC7721 was decreased significantly in the gene transfected group as compared＇ to that of control group. Conclusions The PSUPER-siRNA-IGFIR plasmid can inhibit the growth of human transfected liver-cancer in nude mice.
作者 牛坚 李向农 韩泽广
机构地区 徐州医学院附属医院普通外科 国家人类基因组南方研究中心
出处 《中国普通外科杂志》 CAS CSCD 2007年第8期774-777,共4页 China Journal of General Surgery
关键词 肝肿瘤 RNA干扰 人胰岛素样生长因子1类受体 微血管密度 基因表达 疾病模型 动物 Liver Neoplasma RNA Interference Human Insulin Like Growth Factor Receptor 1 MVD Gene Expression Disease Models, Animal
分类号 R735.7 [医药卫生—肿瘤] R34-33 [医药卫生—基础医学]
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参考文献8

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二级参考文献2

共引文献18

同被引文献29

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中国普通外科杂志
2007年 第8期

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