摘要
目的研究格列美脲对正常大鼠离体心脏缺血预适应(ischemic preconditioning,IPC)保护作用的影响,为临床选择磺脲类药物提供参考。方法Langendorff灌流大鼠离体心脏,随机分为四组:缺血对照组(CON);缺血预适应保护组(IPC);缺血预适应+格列本脲组(IPC+Glb);缺血预适应+格列美脲组(IPC+Glm);记录血流动力学指标,灌流后测心肌梗死面积。结果IPC组和IPC+Glm组心肌梗死面积均较对照组明显减小(P<0.01),IPC+Glb组心肌梗死面积较IPC组增大(P<0.01)。结论IPC使缺血后心肌梗死面积明显减小,格列美脲对IPC心肌保护作用的影响弱于格列本脲。
Objective To explore the effect of Glimepiride on isolated rat heart myocardial protection afforded by ischemic preconditioning. Methods The isolated male Spragur-Dawlay rat hearts were perfused by Langendorff perfusion system, and randomly divided into four groups： ischemia group （CON group） ; ischemic preconditioning group（ IPC group） ; ischemic preconditioning ＋ Glibenclamide （ 10 μM） （ IPC ＋ G1b group） ; ischemic preconditioning ＋ glimeplride（ 10μM） （ IPC ＋ Glm group）. The heamodynamic indexes were recorded during the perfusion and the infarct size of each isolated heart were measured after perfusion. Results Under the conditions of our test, the infarct size of isolated hearts in IPC and IPC ＋ Glm groups were significantly reduced comparing with isolated hearts in CON group（P 〈 0.01 ）, the infarct size of isolated hearts in IPC ＋ Glb group were significantly increased as compared with isolated hearts in IPC group（ P 〈 0.01 ）. Conclusion Ischemic preconditioning significantly reduced the infarct size afforded by sublethal ischemia. Glimepiride dose not abolish the myocardial protection afforded by ischemic preconditioning while glibencalmide can abolish it completely.
出处
《同济大学学报（医学版）》
CAS
2007年第4期29-33,共5页
Journal of Tongji University(Medical Science)
基金
上海市科委科技攻关重大项目(04DZ19507)
