探讨188例乙型肝炎病毒前S2抗原的检测及其临床意义

About detection of 188 patients of pre-S2 antigen of HBV in clinical samples and its clinical significance

目的:探讨乙肝病毒前S2抗原(pre-S2Ag)的检测及其临床意义。方法:对188例标本采用ELISA法进行乙型肝炎病毒标志物(HBVM)及pre-S2Ag检测,并对其中162例标本应用荧光定量PCR法检测HBV-DNA。结果:162例血清标本同时检测HBV-DNA和pre-S2Ag,两者检出率差异无统计学意义(χ2=2.78,P>0.05)。HBeAg(+)与HBeAb(+)组之间pre-S2Ag阳性率的差异有统计学意义(χ2=28.03,P<0.005)。HBeAg(+)组、HBcIgM(+)组pre-S2Ag阳性率为100%,HBsAg(+)的肝癌组pre-S2Ag阳性率达95.0%,结果明显高于HBV-DNA(-)组18.4%,P值均<0.005。结论:pre-S2Ag是反映病毒感染与复制的指标,与临床病情活动有关,可作为疗效和预后的观察指标,能完善和补充乙肝病毒血清标志物的检测。

Objective：To study the detection of the pre-S2 antigen of HBV in clinical samples and its clinical signifieanee.Methods.,HBV M and pre-S2 Ag were detected by Enzyme-linked immunoabsorbent assay （ELISA）in 188 serum samples,and then real-time quantitative PCR were used to detect the HBV-DNA in 162 serum samples of all.Results：Detected the HBV-DNA and pre-S2 Ag in 162 serum samples,there were no differernee in the positive rate （X^2=2.78,P〉0.05）.Between the positive groups of HBeAg and HBeAb,the rate of pre-S2 Ag has significant difference （x^2=28.03,P〈 0.005）.In the groups of HBeAg （＋）and HBeIgM （＋）,the positive rate of pre-S2 Ag was 100%.The pre-S2 Ag was up to 95.0% in the group of hepatic cancer patients with HBsAg positive.The results were remarkably significantly higher than the group of HBV-DNA negative.the pre-S2 Ag was 18.4% positive （P〈0.005, respeetively）.Conclusion：The exist of pre-S2 Ag is associated with virus replication and disease course.It can be used as a direction of treatment and a valuable compensation of the detection of HBV serum makers.