gp150蛋白对盘基网柄菌发育阶段膜蛋白的影响

Influence of gp150 Protein on Cell Membrane Proteins during Dictyostelium Development

用生化抽提细胞质膜蛋白和SDS-梯度聚丙烯酰胺凝胶电泳等方法比较gp150突变AK127细胞和野生型KAx-3细胞质膜蛋白的结果显示,各发育时期的AK127细胞和KAx-3细胞在含量方面比较恒定的质膜蛋白组分是68kD,60kD,54kD,50kD,37kD,34kD和31kD条带,它们并不因为AK127细胞缺失gp150分子而有所改变.发育12h后,蛋白含量变化最为明显的是45kD,25kD,20kD和17kD蛋白,由于同时期突变细胞的这些蛋白条带含量没什么变化,推测这些条带的变化与gp150分子有密切关系;同时野生型细胞还增加了两条10～12kD蛋白条带,这些蛋白可能与gp150分子一样在发育中有表达的时间性和空间性,且与细胞信号传递有密切关系.

To study the change of membrane proteins during Dictyostelium discoideum development, we used wild-type cells KAx-3 and mutant cells AK127. Membrane proteins was prepared by biochemical extraction by TritonX-114, separated using SDS-PAGE and scanned by BIOPROFILE of VILBER LOURMAT. The results showed that 68 kD,60 kD,54 kD,50 kD,37 kD,34 kD and 31 kD are more stable during wild-type cells KAx-3 and mutant cells AK127 developments; they were not changed by AKI27 ceils devoid of gpl50 proteins developments. The difference of protein content exist in 45 kD, 25 kD, 20 kD and 17 kD proteins, contents of those proteins being increased in the wild cells, and two new 10-12 kD proteins appeared in wild cells after 12 h development. This result indicates contents of those proteins expression are influenced by gp150 protein.