大麦黄矮病毒GPV株系运动蛋白基因原核表达载体的构建

Construction of the prokaryotic expression vector of move protein gene of Barley yellow dwarf virus GPV

大麦黄矮病毒GPV株系ORF4基因编码一种17 kDa的蛋白,利用一对特异性引物,通过RT-PCR对ORF4基因进行了扩增,序列测定表明其长度为456 bp,与已报道的序列一致,并构建了原核表达载体。

A 17 kDa protein were encoded by ORF4 gene of Barley yellow dwarf virus GPV. ORF4 gene was amplified by RT-PCRrnethod and sequenced. The nucleotide sequence indicated that ORF4 had 456 bp nucleotides. Prokaryotic expression vector of move protein gene of Barley yellow dwarf virus GPV was construct.