摘要
研究了忍冬诱导生芽、增殖培养、诱导生根的培养基及配方,结果表明MS+6-BA1.0mg/L+NAA0.1mg/L是适宜的生芽诱导培养基,MS+6-BA1.5mg/L+NAA0.01mg/L、MS+6-BA1.5mg/L+NAA0.05mg/L是适宜的增殖培养基,1/2MS+NAA2.5mg/L+活性炭200mg/L是较适宜的生根培养基。并为其它金银花组培快繁提供技术借鉴参数。
the Rapid Propagation Technology of Tissue Culture for Lonicera japonica Thunb. were studied by adopting different culture mediums and formulae of shoot induction, proliferation and rooting .The results showed that the optimum culture medium for shoot induction was MS＋6-BA1.0mg/L＋ NAA0.1 mg/L; MS＋6-BA1.5mg/L＋NAA0.01 mg/L, MS＋6-BA1.5mg/L＋NAA0.05 mg/L was proliferation medium. It could still form a good root system in a 1/2MS＋ NAA2.5 mg/L＋ active carbon 200mg/L medium. This would provide some useful parameters for reference in other Lonicera japonica.
关键词
金银花
组培快繁
培养基及配方
Lonicera japonica Thunb
Rapid propagation via tissue culture
mediums and formulae
