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酵母三杂交系统的原理和应用 被引量：2

Principle and application of the yeast three-hybrid system
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摘要 酵母双杂交系统自出现以来,广泛用于研究蛋白质之间的相互作用,它是一种具有高灵敏度的研究蛋白质之间关系的技术。在酵母双杂交系统基础上发展的酵母三杂交系统将应用范围扩展到蛋白质-蛋白质、蛋白质-RNA、蛋白质-小分子化合物等更广阔的研究领域。本文着重介绍酵母三杂交系统的原理、应用及局限性。 The yeast two-hybrid system is widely employed in genetic screening and detecting protein-protein interactions. The yeast three-hybrid system, based on the yeast two-hybrid system, can be applied to much broader areas for detection of interactions between protein-protein, protein-RNA and protein-small ligand. This minireview deals with the principle, application, and shortcoming of the yeast three-hybrid system.
作者 彭丹妮 黄静 吴自荣
机构地区 华东师范大学生命科学学院
出处 《生命科学》 CSCD 2007年第4期461-464,共4页 Chinese Bulletin of Life Sciences
基金 上海市科委2004年重大计划项目(04DZ19207-1)
关键词 酵母三杂交系统 蛋白质-蛋白质相互作用 蛋白质-RNA相互作用 蛋白质-小分子化合物相互作用 yeast three-hybrid system protein-protein interactions protein-RNA interactions proteinsmall ligand interactions
分类号 Q-33 [生物学]
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参考文献18

  • 1Fields S,Song O.A novel genetic system to detect proteinprotein interactions.Nature,1989,340(6230):245-246.
  • 2Sengupta D J,Zhang B,Kraemer B,et al.A three-hybrid system to detect RNA-protein interactions in vivo.Proc Natl Acad Sci USA,1996,93(16):8496-8501.
  • 3Zhang J,Lautar S.A yeast three-hybrid method to clone ternary protein complex components.Anal Biochem,1996,242(1):68-72.
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同被引文献14

  • 1吴娟,钱凯,杨泽峰.酵母双杂交系统的研究进展[J].安庆师范学院学报（自然科学版）,2005,11(2):59-63. 被引量：8
  • 2张莉,黄倢.酵母双杂交系统的改进与研究进展[J].海洋湖沼通报,2006(1):108-116. 被引量：5
  • 3Fields S., Song O.. A novel genetic system to detect proteinprotein interactions. Nature, 1989,340(6230):245--246.
  • 4Vidal M.,Braun P.,Chen E.et al. Genetic characterization of a mammalian protein2protein interaction domain by using a yeast reverse two2hybrid system.Proc Natl Acad Sci USA, 1996, 93: 10321 - 10326.
  • 5Hirst M., Ho C., Sabourin L. et M. A two-hybrid system for transactivator bait proteins.Proc Natl Acad Sci USA, 2001,98: 8726--8731.
  • 6Aronheim A.,Zandi E.,Hennemann.et al. Isolation of an AP-1 repressor by a novel method or detecting protein-protein interactions.Mol Cell Biol,1997,17:3094--3102.
  • 7Isakoff S.J.,Cardozo T.,Andreev J.et al.Identification and analysis of PH domain-containing targets of hosphatidylinositol3- kinase using a novel in vivo assay in yeast.EMBO J, 1998,17: 5374--5387.
  • 8Zhang J., Lautar S.. A yeast three-hybrid method to clone ternary protein complex components. Anal Biochem, 1996, 242 (1):68--72.
  • 9Sengupta D.J.,Zhang B.,Kraemer B.et al.A three-hybrid system to detect RNA-protein nteractions in vivo.Proc Natl Acad Sci USA,1996,93(16): 8496--8501.
  • 10Licitra E. J., Liu J. O. A three-hybrid system for detecting small ligand- protein receptor interactions. Proc Natl Acad Sci USA,1996,93(23): 12817-- 12821.

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生命科学
2007年 第4期

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