弧菌显色培养基进行多种弧菌分离鉴定的研究

Isolation and identification of Vibrio in marine products by Vibrio chromogenic medium

目的：应用弧菌显色培养基进行多种弧菌分离的可行性.方法：对120份海（水）产品经碱性蛋白胨水增菌后接种分离于科玛嘉弧菌显色培养基（CV）,37℃×18～20 h培养后观察各菌的生长情况,挑选各种颜色菌落纯培养后由微生物鉴定药敏分析仪鉴定其菌属.结果：在海（水）产品的多种弧菌分离鉴定中,不同的弧菌在CV上显示不同的颜色.结论：易于观察,操作简单,大大地减少了工作量和试剂的消耗量,适合在基层推广使用.

Objective：The feasibility to isolate many type of Vibrio by Vibrio chromogenic medium was studied. Methods：Mter bacterial enrichment in alkaline peptone water, 120 samples of marine products were inoculated in CHROMager Vibrio colored medium （CV） and incubated at 37℃ for 18 -20 h. Following this, bacterial colony with different color was chosen to purify according to its growth and character, and identified by microbiology identification and antibiotic susceptibility analysis instrument. Results：In the isolation and identification of Vibrio in marine products, different type of Vibrio in CHROMager Vibrio colored medium （CV） showed different color. Conclusion：The method is simple and the result is observed easily. Moreover, the method can reduce a great deal of workload and wastage of reagent, so it is suitable for application in grass roots.