71株肠道病原菌的耐药性及对小鼠的毒力研究

Study of drug resistances in 71 strains enteropathogens and toxicity to mouse

目的：了解71株肠道病原菌的耐药性及对小鼠的致病力.方法：用血清学、API生化对病原菌进行鉴定,用药物敏感性试验进行耐药性监测,用小鼠毒力试验研究肠道病原菌的致病力.结果：志贺菌鉴定必须用血清学、生化学相互补充进行鉴定,肠道病原菌对头孢噻肟敏感率为100%,对环丙沙星敏感率为95.8%,阿莫西林敏感率为91.5%;对奈啶酸耐药率为57.7%;鲍氏志贺菌12-15型、16-18型对小鼠无毒力,其它病原菌均可引起小鼠肠、胃、心脏、肝脏病变,并在12～48 h内致小鼠死亡.结论：由肠道病原菌引发的疫情暴发或流行,首先选用头孢塞肟、环丙沙星、阿莫西林进行临床治疗用药或预防性服药,鲍氏志贺菌的致病性有待进一步研究.

Objective：To investigate the drug resistances of 71 strains enteropathogens and pathogenicity to mouse. Methods： Identification of pathogens was performed by serology, API biochemical test. Drug resistance was monitored by drug sensitivity test. pathogenicity was used by mouse toxicity test. Results：Shigella identification must be tested by serology, chemistry complement each other. Sensitivety rates of cefotaxime, Ciprofloxacin and amoxicillin were 100% , 95.8%, 91.5%, respectively. For nalidixic acid, the drug resistance rate was 57.7%. 12 - 15 and 16 - 18 type Bohler Shigella were nontoxic to mice while other enteropathogens can cause mouse intestine, stomach, heart, liver lesions, and in 12 - 48 hours induced death. Conclusion： Treatment or preventive medication of enteropathogens caused outbreak or epidemic should be first select cefotaxime, Ciprofloxacin and amoxicillin. Pathogenicity of Bohler shigella should be further studied.