保卫细胞中特异表达启动子片段缺失后表达载体的构建

Cloning and Construction of Expression Vector of Dissected Guard Cell-specific Gene Promoters

iMyAP基因启动子的核心启动子370 bp片段能启动基因在保卫细胞中特异性表达。将其进行缺失,克隆得到370 bp、308bp和150 bp的启动子片段,与pMD18-T载体连接并进行序列分析后用BLAST软件作同源分析的结果显示,它们的核苷酸序列与GenBank中登陆的iMyAP启动子片段的核苷酸序列一致。将这3个启动子片段分别与pCAMBIA-1303质粒相连,构建得到表达载体。

The 370bp core promoter of iMyAP gene can promote gene express specifically in the guard cell.Three fragments（the lengths of them were 370 bp,308 bp and 150 bp,respectively） were cloned and inserted into pMD18-T vector.The result showed that the sequences inserted into pMD18-T vectors were identical with the sequences published in GenBank.Then the fragments were connected with pCAMBIA-1303 plasmid and the expression vectors were constructed.