人巨细胞病毒对小鼠宫颈的诱癌作用

The carcinogenesis of human cytomegalovirus to mouse cervix

用眼科镊将浸透了病毒母液的明胶海绵块塞入小鼠阴道，直抵宫颈的办法，将紫外线灭活的人巨细胞病毒（ＨＣＭＶ）投给昆明种杂交小鼠。约３１周后，有１５０％小鼠诱发了实验性宫颈癌，另一组加用巴豆油促癌剂，小鼠宫颈癌发率为１８８％，对照组癌发率为０。ＨＣＭＶ诱癌组１２例小鼠宫颈癌组织ＨＣＭＶＩＥ（ｉｍｍｅｄｉａｔｅｅａｒｌｙ）抗原均为阳性，血清ＨＣＭＶＩＥ抗体几何平均滴度为１∶１６７２，对照组以上两个指标均为阴性。ＨＣＭＶ诱癌组小鼠外周血淋巴细胞ＡＮＡＥ阳性率为３８９±６８％，对照组为６７８±８０％。

We plugged the gelfoam impregnated with virus stock solution into mouse cervix with ophthalmic forceps. Mice of Kun Ming strain were infected with UV inactivated HCMV (human cytomegalovirus). After 31 weeks, cervical carcinomas were induced in 15% of the infected mice. In another group, infected with UV-inactivated HCMV adding croton oil, the carcinogenesis was 18.8%, but incontrol group, it was 0%. HCMV IE(immediate early) antigen in 12 cancer tissues of UV inactivated HCMV treated group were all positive, and HCMV IE antibody geometrical average titre in mouse serum of the same group was 1∶167 2, but the same two parameters in control group were both negative. The ANAE positive rate of lymphocytes in mouse peripheral blood of UV inactivated HCMV treated group was 38 9±6 8%, and of control group was 67 8±8 0%.