HIV感染者血清中4种微量元素含量分析

Study on serum level of four trace elements in HIV-infected persons

目的了解人类免疫缺陷病毒(HIV)感染后对机体血清Zn、Fe、Cu、Mn 4种微量元素的影响,为今后对HIV感染者采取合理营养治疗提供依据。方法随机抽取用酶联免疫吸附试验(ELISA)筛查、免疫印迹法确认的成年无症状HIV感染者50例,采用电感耦合高频等离子体原子发射光谱仪测定其血清中锌(Zn)、铁(Fe)、铜(Cu)、锰(Mn)4种微量元素含量。结果与正常值相比,HIV感染者血清中Zn、Fe含量明显下降(P<0.01),而Cu、Mn含量则显著上升(P<0.01)。结论尽管HIV感染者尚未出现明显艾滋病相关综合征,但血中原有的微量元素平衡已遭破坏。这可能是HIV感染者健康损伤的早期表现。提示对HIV感染者应尽早采取营养监测与干预措施。

Objective To observe serum trace element（Zn, Fe, Cu, Mn） levels in HIV - infected adults, which provided a research basis for nutritional therapy to HIV-infected person. Methods 50 blood samples from HIV - infected adults that confirmed by enzyme linked immunosorbent assay and immunoblotting methods were collected in random. The contents of four kind of microelement （Zn, Fe, Cu, Mn） in serum were determined by inductively coupled plasma and atomic emission spectrum （ ICP - AES）. Results Zn and Fe levels in serum of HIV - infected adults were significantly lower than normal level （P 〈 0.01）. But Cu and Mn levels were obviously higher than normal level （ P 〈 0.01 ）. Conclusion Though some syndromes correlating with AIDS did not occur to HIV-infected person, the balance of serum microelement level was broken down. The results indicated that serum microelement levels were corrected with the pathogenetic of HIV-infected person. The balance destruction of microelement levels maybe a nonage reflection to HIV-infected person＇s health impairment. Nutritional surveillance and intervention should be done early to HIV-infected person.