HIV感染者抗病毒治疗及性行为情况凋查

Investigation on HAART of HIV infection and sexual behaviors

目的研究河南省高效抗逆转录病毒治疗效果和耐药情况,为防止耐药株流行提供理论依据。方法采用问卷调查方法,了解人类免疫缺陷病毒(HIV)感染者人口学、治疗和漏服情况及安全套使用情况,RT-PCR和套式PCR扩增HIV pol区基因,双脱氧法测定逆转录酶和蛋白酶基因序列,与国际耐药数据库比对辨别耐药变异。结果本次调查人群文化程度较低,家庭经济条件差;治疗组病毒载量显著低于未治疗组(P<0.01);安全套使用率低(23.1%);服药依从性较差(72.2%):治疗人群中发现6例存在耐药突变,未治疗感染者发现1例存在耐药变异。结论抗病毒治疗初期疗效显著,耐药变异率低,但漏服和较低的安全套使用率将导致耐药变异快速产生和流行,应加强用药督导和安全套普及。

Objective To prevent the prevalence of drug resistance viruses, drug resistance muations of HIV- 1 and curative effect after taking HAART among HIV - 1 infected individuals in Henan province were analyzed. Methods Patients＇ information about demology, treatment, compliance and condom were acquired from questionnaires, amplified HIV-1 pol gene by RT-PCR and nest-PCR, sequenced reverse trascraptase （RT） and protease regions and compared with sequence in Stanford HIV drug resistance sequence database. Results The education level of patients and the income investigated was lower; The viral load in treated patients was obviously lower than that in untreated patients （P 〈 0.01 ） ; Lower frequency in condom use （23.1%） ;frequency of patients who followed doctors was lower（72.2 % ）;six drug resistance mutations were found in six treated patients, and one drug resistance mutations were found in untreated patients. Conclusion The curative effect was notable in early period of HAART, The frequency of drug mutation was lower, but lower condom use and disobedience of taking drug would make drug mutations occur and spread. To prevent the prevalence of drug resistance viruses, monitoring the drug taking and generalizing the condom were necessary.