纳米掺锌羟基磷灰石的制备及抗菌性能

Preparation and Antibacterial Properties of Zinc-Doped Hydroxyapatite Nanoparticles

采用溶胶-凝胶-超临界CO2干燥法,制备了羟基磷灰石(HAP)和Zn/[Zn+Ca]摩尔比γ为0～0.2的掺锌羟基磷灰石(ZnHAP)的纳米微粒.通过FTIR、XRD、TEM分析了锌的掺入对HAP结构、晶形及结晶度的影响;通过抑菌环和抗菌率实验,研究了HAP掺锌后大肠杆菌、金黄色葡萄球菌、乳酸杆菌的抗菌性能.结果表明:采用溶胶-凝胶-超临界CO2干燥法,可制备纳米HAP和纳米ZnHAP,锌可以部分取代钙掺入HAP的结构中;HAP掺入锌后其红外光谱特征吸收峰的振动频率增大,但吸收峰的强度及结晶度随γ增大而降低,晶形从短棒状变为针状;当试样抗菌浓度为0.1 g/mL时,纳米HAP及γ在0.04以下的纳米ZnHAP仅在动态作用下有抗菌作用;而γ在0.08以上时,无论是在静态还是动态作用下ZnHAP都具有抗菌作用,且抗菌性能随掺锌量的增加而提高.说明掺锌可以改善HAP的抗菌性能.

Hydroxyapatite （HAP） nanoparticles and zinc-doped hydroxyapatite （ZnHAP） nanoparticles with a Zn/[ Ca ＋Zn] molar ratio γ of 0-0. 2 were prepared by means of sol-gel-supercritical fluid drying. Then, FTIR, XRD and TEM, XRD were adopted to reveal the effects of zinc doping on the structure, crystal shape and crystallinity of the prepared nanoparticles. Moreover, the bacterial inhibition zone and antibacterial ratio were tested to investigate the antibacterial properties of HAP and ZnHAP nanoparticles to Escherichia coli, Staphylococcus aureus and Lactobacillus. The results are as follows. The sol-gel-supercritical fluid drying is an effective method to prepare HAP and ZnHAP nanoparticles; zinc can be partially doped in HAP as the substitute of Ca to form ZnHAP; after the doping of zinc, the vibration frequencies of IR bonds in ZnHAP increase, the transmittance and the crystallinity decrease with the γ value increasing, and the morphology of the nanoparticles changes from short rod shape to needle shape ; both the HAP nanoparticles and the ZnHAP nanoparticles with a γ value of less than 0. 04 possess antibacterial properties only in dynamical forcible contact with tested bacteria at a nanoparticles content of 0. 1 g/mL; the ZnHAP nanoparticles with a γ value of more than 0. 08 possess an antibacterial ability which increases with γ value at the same content no matter whether it is in static or dynamic contact with the tested bacteria. All of the results indicate that the antibacterial property is improved after the zinc doping.