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风信子AGL6同源基因在拟南芥中异位表达引起提早开花和器官同源转化 被引量:7

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摘要 本文对风信子(Hyacinthus orientalis L.)与拟南芥(Arabidopsis thaliana)AGL6的同源MADS盒基因HoAGL6进行了功能研究.基因表达分析结果显示,HoAGL6 mRNA在花序芽、花被、心皮和胚珠中积累,但未出现于雄蕊、营养器官的叶和鳞片中.这说明HoAGL6参与了风信子花的形成,以及花被和子房的发育.HoAGL6在拟南芥(Arabidopsis thaliana)中异位表达,转基因植株表现为株型矮小,极度提早开花,并失去花序的不确定性.因此,HoAGL6参与了开花时间调节.另外,35S::HoAGL6转基因拟南芥植株频繁出现器官同源转化,包括花萼、花瓣和叶片转化为心皮状结构或子房结构,且雄蕊缺失或数目减少.此结果表明,在转化植株中HoAGL6参与了花器官特性决定.进一步的研究表明,在35S::HoAGL6的拟南芥转化植株中显著提高了开花时间调节基因SOC1和花分生组织特征基因LFY的表达水平,并且HoAGL6的异位表达也在叶片中激活了花器官特征基因AG和SEP1的表达.这些结果支持在拟南芥中HoAGL6通过促进SOC1和LFY的表达来调节开花时间,以及通过激活AG和SEP1参与了花器官发育.
出处 《中国科学(C辑)》 CSCD 北大核心 2007年第4期466-478,共13页 Science in China(Series C)
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