摘要
通过单因素实验确定实验对象与水平,根据Box-Benhnken的中心组合实验设计原则,在单因素试验的基础上采用3因素3水平的响应面分析法,建立了多糖提取率与各影响因子的回归方程,并得到以双孢菇多糖提取率为响应值的响应面图和等高线图,进而分析得出了双孢菇多糖提取的最佳工艺条件:提取温度71.8℃、提取时间125 min,料液比1∶31.3,提取次数1次,在此条件下双孢菇多糖的理论提取率可达1.72%.
Experiment factors and levels of extraction of Agaricus bisporus polysaccharide（ABPS） were selected by single factor tests; and according to the Box-Benhnken center united experimental design principles and based on the single factor tests, the method of response surface analysis with 3 factors and 3 levels was adopted; the regression equation on factors influencing the parameters was established; response surface graph and contour graph with the extraction rate response value of extracting ABPS were obtained; and the optimum conditions are as follows; extraction temperature 71.8;C ; extraction time 125 mini ratio of material to water 1：31.3; and that the extraction rate of ABPS is up to 1.72%with once extraction.
出处
《三峡大学学报（自然科学版）》
CAS
2007年第4期367-370,共4页
Journal of China Three Gorges University：Natural Sciences
关键词
双孢菇
多糖
提取
优化
Agaricus bisporus
polysaccharides
extraction
optimization
