梨新品种及其亲本的AFLP分析

AFLP Analysis of New Pear Cultivars and Their Parents

利用荧光AFLP技术,对20个梨新品种及其23个亲本(共43个品种)进行研究。从64对引物组合中筛选出7对用于扩增,共获得784条带,其中多态性带699条,多态性为89.2%。扩增结果显示,7对引物组合在29个品种中扩增出特征带,每对引物组合均能将所有品种鉴别开,表明荧光AFLP技术用于梨品种鉴定的效率很高。通过聚类,从分子水平对梨新品种及其亲本的遗传关系进行分析,并对20个梨新品种进行分类,为梨杂交育种的亲本选配提供了理论参考。

Forty-three pear cuhivars including 20 new pear cuhivars and their parents were subjected to fluorescent AFLP （amplified fragments length polymorphism） analysis. Seven primer combinations selected from 64 primer combinations produced a total of 784 fragments, of which 699 were polymorphic with a polymorphism percentage of 89.2%. The results showed that characteristic bands were amplified from 29 cultivars, and any primer combination could identify all the cuhivars. Fluorescent AFLP was proved to be a very reliable method to identify pear cuhivars. The genetic relationship between new pear cultivars and their parents was analyzed and 20 new cultivars were classified on molecular level by clustered analysis, which provided theoretical reference for parent selection in pear hybridization breeding.