乐昌含笑组织培养过程中根的诱导

Root Induction During Tissue Culture of Michelia chapensis Dandy

以乐昌含笑为材料,采用6-苄氨基嘌呤(6-BA)、萘乙酸(NAA)、吲哚丁酸(IBA)三因素二次正交旋转组合设计,对组织培养过程中根的诱导进行了3种生长调节剂不同配比的对比试验。结果表明:在23个组合中,较低6-BA、NAA和IBA的浓度能促进乐昌含笑根的诱导和生长;外植体在1/2MS+6-BA0.40mg·L-1+IBA0.60mg·L-1+NAA0.80mg·L-1培养基上出根率和生根数分别达到50%和1.5条以上,高于其它处理,而且根系生长良好。

Effects of 6-BA, NAA and IBA alone or combination at various concentrations on the root induction of Michelia chapensis Dandy during tissue culture were investigated. The results showed that low concentrations of 6-BA, NAA and IBA were suitable for the root induction and growth of Michelia chapensis Dandy. The root formation rate was more than 50%, while the root number of each plant was more than 1.5 with grown planflets when the explants were transferred to the 1/2MS medium containing 0. 40 mg· L^-1 6-BA and 0. 60 mg· L^-1IBA and 0. 80 mg· L^-1 NAA. Meanwhile, it was observed that the root developed well.