常温超高压提取大花紫薇叶中2α-羟基熊果酸

Study on Super-high-pressure Extraction of 2α-Hydroursolic Acid from Lagerstroemia speciosa(L.) Pers. Leaves

在单因素试验的基础上,采用正交试验法对2α-羟基熊果酸的提取工艺进行优选,选用L9(34)进行正交试验,以2α-羟基熊果酸的得率为指标,考察常温(25℃)下超高压压力、保压时间、乙醇体积分数及其固液比(g∶mL)对2α-羟基熊果酸得率的影响。得到的最佳工艺条件为超高压压力300 MPa,保压时间5 min,乙醇体积分数90%,固液比1∶15,提取3次。2α-羟基熊果酸的提取得率可达0.415%。

The super-high-pressure （SHP） extraction of 2α-hydroursolic acid from leaves of Lagerstroemia speciosa （Linn.） Pers. was studied. The optimum extraction condition was optimized with orthogonal test by using L9 （34） orthogonal design with extractive yield of 2α-hydroursolic acid as index, investigating the influences of SHP pressure, time, ethanol concentration and the ratio of solid to liquid. Optimum extraction conditions were determined as follows ： SHP pressure 300 MPa, SHP time 5 rain, 90 % ethanol as extracting solvent, stock ratio 1 ： 15 （g： mL）, extracting for three times. The yield of 2α-hydroursolic acid was 0. 415 %.