摘要
目的从神经递质角度探讨褪黑素(melatonin,Mel)抗癫痫作用的机制。方法采用匹罗卡品(pilocarpine,PILO)诱发大鼠癫痫持续状态(status epilepticus,SE)模型,观察大鼠SE后4个时相点即6、48、72h和7d海马GABA含量和GAD67mRNA表达的变化,以及Mel对其变化的影响。结果PILO诱导大鼠SE后6h至7d,海马GABA含量显著降低,与对照组比较差异极显著(P<0.01),GAD67mRNA的表达于SE后6~72h较对照组明显降低(P<0.01),SE后7d,GAD67mRNA的表达升高并明显高于对照组(P<0.05);PILO+Mel组大鼠SE后6h至7d,海马GABA水平显著高于PILO组(P<0.01),并且GAD67mRNA的表达在SE后6~72h,也显著高于PILO组大鼠(P<0.05)。结论Mel可能通过上调GAD67mRNA的表达,使GABA合成增加,抑制功能增强来发挥抗癫痫作用。
Objective To probe the mechanism of anticonvulsant by melatonin from the angle of neurotransmitter. Methods Rat status epilepticus (SE) model was induced by pilocarpine(PILO). γ-aminobutyric acid (GABA) content and glutamin acid decarboxylase (GAD)67 mRNA expression was detected at 6, 48, 72 h, and 7 d in the hippocampus of post-SE rats. The effect of melatonin on these changes was observed. Results GABA content was significantly lower in the hippocampus than in control( P 〈 0. 01 ). At 6 -72 h post- SE, the expression of GAD67 mRNA was significantly lower in PILO group than in control(P 〈 0. 01 ) , but significantly higher in PILO group than in control at 7 d post-SE. The content of GABA was significantly higher in PILO + Mel group than in PILO group at 6 h -7 d post-SE(P 〈0. 01 )and the expression of GAD67 mRNA was significantly higher in PILO + Mel group than in PILO group at 6 - 72 h post-SE ( P 〈 0. 05 ). Conclusion Anticonvulsive effect of melatonin may be due to its ability to increase the expression of GAD67 mRNA, so to increase the content of GABA.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第16期1605-1608,共4页
Journal of Third Military Medical University
关键词
癫痫持续状态
海马
褪黑素
Γ-氨基丁酸
谷氨酸脱羧酶
status epilepticus
hippocampus
melatonin
γ-aminobutyric acid
glutamin acid decarboxylase