便秘大鼠结肠黏膜上调蛋白质的分离及鉴定

Separation and identification of up-regulated proteins in colonic mucosa from chronic slow transit constipation rats

目的观察慢传输型便秘大鼠结肠黏膜上调蛋白质的变化特性,并对明确的蛋白质组进行功能分析。方法利用复方苯乙哌啶建立大鼠慢传输型便秘模型,利用高分辨双向电泳(2-DE)对STC大鼠结肠黏膜组织进行蛋白质分离,ImageMaster2D Elite图像分析软件进行分析,应用生物质谱、蛋白质库及文献分析等技术对差异蛋白质中的上调蛋白质组进行鉴定、功能和临床意义分析。结果慢传输型便秘在其发生过程中有显著的蛋白质表达差异。主要差异表达的蛋白质有:类肌钙蛋白Calponin(CaP)(A1),组蛋白H2B(A2),鼠醛酮还原酶蛋白类似物(RAKb蛋白类似物)(A3),A1、A2、A3在便秘大鼠的胶图中表达明显上调。结论CaP与胃肠道的运动状态密切相关,它在胃肠道运动中具有调节作用,可能介导了复方苯乙哌啶的抑制胃肠动力作用,通过表达量的变化来实现对胃肠道运动的调节。

Objective To investigate the general changes of up-regulated proteins in colonic mucosa from chronic slow transit constipation rats and analyze the function of the specified proteins. Methods An experimental rat model of chronic slow transit constipation （STC） was established by diphenoxylate and used for separating proteins from colonic mucosa by two-dimensional electrophoresis （2DE）. ImageMaster 2DElite, mass spectrometry, protein bank and bibliometrics were applied to identify the up-regulated proteins and analyze their clinical significance and function. Results Obvious distinction was observed in the genesis of STC, such as calponin （ CaP, A1 ）, histone H2B （A2） , rat aldoketo-reductase analog （ A3 ）. The expression of A1, A2, and A3 up-regulated in the gel graph of STC rats. Conclusion Calponin plays a vital role in regulating the gastrointestinal movement by amount alteration and mediates the effect of gastrointestinal dynamia of diphenoxylate.