摘要
目的对应用cDNA芯片技术筛选出的一条全长胰腺癌相关新基因进行鉴定。方法对应用cDNA芯片技术筛选出的一条全长胰腺癌相关新基因进行测序和生物信息学分析,应用RT-PCR和Northern blot检测该基因在12例胰腺癌和癌旁正常胰腺组织以及4种胰腺癌细胞株中的表达情况。结果生物信息学分析显示新基因定为于染色体4p15,有一包含501bp的ORF,拟编码由166个氨基酸组成的蛋白质,其理论分子量为18293.23,等电点为8.57。BLASTp分析发现,该蛋白与一种人类假想蛋白FLJ90013同源性大于90%,可能为该假想蛋白家族新成员。RT-PCR显示新基因在12例胰腺癌组织和4种胰腺癌细胞株中均表达,而在正常胰腺组织中未见表达。Northern blot分析得到相同的结果。结论新基因与胰腺癌的发生、发展密切相关,可作为胰腺癌诊断和基因治疗的分子靶标。
Objective To identify a novel full-length human pancreatic cancer-associated gene screened by eDNA microarray. Methods One novel full-length gene which overexpressed in the pancreatic cancer was sequenced and analyzed for bioinformatics. The expression of the novel gene in 12 specimens of pancreatic cancer and normal tumor-surrounding pancreatic tissues and 4 pancreatic cancer cell lines were detected by RTPCR and Northern blot analysis. Results Bioinformaties analysis showed that the ehromatosome of the novel gene localized to 4p15, and had an open frame of 501 base pair encoding 166 amino acids of protein. Its theoretical molecular weight was 18 293.23 and PI was 8.57. This protein had more than 90% homologous human hypothetical protein FLJ90013 using BLASTp analysis, and may be a new member of FLJ90013 protein family. The novel gene expressed in 12 specimens of pancreatic cancer tissues and 4 pancreatic cancer cell lines, but not in normal pancreatic tissues. This result was just the same as the result of Northern blot analysis. Conclusion The analysis of gene expression profile of tumor based on eDNA mieroarray can realize high-throughput screening of the genes associated with the pancreatic cancer, and it can help to rapidly explore the gene function. The novel gene is associated with oneogenesis and development of pancreatic cancer, and might be potential tumor marker or molecular target for diagnosis and treatment of pancreatic cancer.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第17期1709-1711,共3页
Journal of Third Military Medical University
关键词
胰腺癌
CDNA芯片
基因表达谱
pancreatic cancer
eDNA microarray
gene expression spectrum