蒿甲醚对紫外线照射后HaCaT细胞表达Fas和Bcl-2的影响

Effect of Artemether on the Expression of Fas and Bcl-2 in HaCaT Cells after Exposure to UV

目的为探讨一定剂量不同波长紫外线照射HaCaT细胞后,不同浓度蒿甲醚对HaCaT细胞表达凋亡相关蛋白Fas和Bcl-2的影响.方法采用45.00 mJ/cm2剂量的UVB及4.00 J/cm2剂量的UVA照射培养的HaCaT细胞(永生化角质形成细胞),以羟氯喹及不同浓度的蒿甲醚进行干预处理,应用流式细胞仪检测紫外线辐照及羟氯喹和蒿甲醚处理后HaCaT细胞Fas和Bcl-2蛋白水平的变化.结果(1)与未照射组相比UVA、UVB照射均可使HaCaT细胞表达Fas增加;(2)与UV组相比UVA加羟氯喹,UVB、UVA加25μg/mL蒿甲醚组使HaCaT细胞表达Fas减少;(3)与未照射组相比UVA、UVB照射可使HaCaT细胞表达Bcl-2减少;(4)与UV组相比UVA加25μg/mL蒿甲醚组和UVB加100μg/mL蒿甲醚组可以使HaCaT细胞表达Bcl-2增加.结论小剂量(25μg/mL、50μg/mL)蒿甲醚与凋亡相关的Fas和Bcl-2的表达有关.

Objective To approach the effect of artemether with different concentrations on the expression of Fas and Bcl -2 in HaCaT cells after exposure to UV which is of invariably dosage and different wavelength. Methods Subconfluent HaCaT cells were shammed or irradiated with 4. 00 J/cm^2 of UVA or 45.00 mJ/cm^2 of UVB respectively. The level of Fas and Bcl -2 protein in HaCaT cells, which were irradiated by Ultraviolet and treated with hydroxychloroquine and different concentrations of artemether, were detected by flow cytometry. Results （ 1 ） Compared with cells without UV irradiation, the expression of Fas was upregulated in HaCaT cells after UVA and UVB irradiation; （2） Compared with cells with UV irradiation, the expression of Fas downregulated in UVA combined with hydroxychloroquine groups, UVB combined with 25 μg/mL of artemether （ H2 ） groups and UVA combined with 25 μg/mL of artemether （H2） groups ; （3） Compared with cells without UV irradiation, the expression of Bcl -2 was downregulated in HaCaT cells after UVA and UVB irradiation; （4） Compared with cells with UV irradiation, the expression of Bcl -2 in HaCaT cells upregulated in UVA combined with H1 groups and UVB combined with 100 μg/mL of artemether （H3） groups. Conclusion Low dosage （25 μg/mL and 50 μg/mL） of artemether could prevent apoptosis of HaCaT cells, and it has protective effect on HaCaT cells damaged from UV irradiation. Artemether might affect the expression of Fas and Bcl - 2 protein, and then reduce UV - induced apoptosis of HaCaT cells.