阿托伐他汀对大鼠血管平滑肌细胞分泌基质金属蛋白酶-1的影响

Effects of Atorvastatin on matrix metalloproteinase-1 secreting by rat vascular smooth muscle cells

目的观察阿托伐他汀对大鼠血管平滑肌细胞分泌基质金属蛋白酶-1(MMP-1)的影响。方法培养的大鼠胸腹主动脉血管平滑肌细胞中先加入10 ng/ml的白细胞介素-1β(IL-1β),24 h后加入不同浓度的阿托伐他汀,继续孵育48 h后,采用酶联免疫吸附法(ELISA法)检测培养上清液中MMP-1的浓度,另外,取阿托伐他汀1×10-5mmol/L浓度,分别在6、244、8 h用同样的方法测培养液上清中MMP-1的浓度。结果随着给药浓度的增加(1×10-7mmol/L、1×10-6mmol/L、1×10-5mmol/L),阿托伐他汀对IL-1β介导的大鼠血管平滑肌细胞分泌MMP-1的抑制作用逐渐增强(加药组MMP-1分泌较对照组分别减少5.18%、11.78%、29.80%),与对照组有统计学差异;随着药物作用时间的延长(6 h、24 h、48 h),阿托伐他汀(1×10-5mmol/L)对大鼠血管平滑肌细胞分泌MMP-1的抑制作用逐渐增强(加药组MMP-1分泌较对照组分别减少17.66%、22.84%、29.80%),与对照组有统计学差异。结论阿托伐他汀呈时间剂量依赖性抑制IL-1β介导的大鼠血管平滑肌细胞分泌MMP-1。

Objective To investigate the effects of atorvastatin on matrix metalloproteinase-1（MMP-1） secreting by rat vascular smooth muscle cells.Methods Rat aortal smooth muscle cells were first incubated in serum-free medium with 10 ng/ml IL-1β;24 hours later,atorvastatins with different concentration（1×10^-7mmol/L,1×10^-6mmol/L,1×10^-5mmol/L） were added to the medium.48 hours later,supernatants of different cultures were collected and MMP-1 levels were determined by ELISA kits according to the manufacturer＇s instructions.In the group with atorvastatin concentration of 1×10^-5mmol/L,MMP-1 levels were respectively determined after 6,24 and 48 hours.Results Atorvastatin inhibited the secretion of MMP-1 in a time-dose-dependent manner.Conclusions Atorvastatin can inhibit MMP-1 secreting by IL-1β stimulated rat vascular smooth muscle cells.