反相高效液相色谱法测定金银花中绿原酸的含量

Determination of the Chlorogenic Acid in Flos Lonicerae with HPLC

为了研究金银花质量控制的有效手段。通过建立反相高效液相色谱法测定了金银花中绿原酸的含量。测定条件为：色谱柱为Nucleosil C18柱（4．6×250m，5μm），流动相为甲醇-0.4％磷酸（体积比为25：75），流速1.0ml／min，光电二极管阵列检测器，检测波长327am，柱温25oC。绿原酸在0.64-6.40μg范围内其进样量与峰面积呈良好的线性关系（r=0.9996），平均回收率为99．3％，相对标准偏差（RSD）为0.8％。以6种不同浓度（0％-95％）的乙醇溶液为提取剂，对绿原酸的提取率进行考察的结果表明，30％乙醇溶液的绿原酸提取率最高，且杂质峰的数量明显减小。该方法具有简便、快速，回收率、精密度高的优点，可作为金银花质量控制的有效手段。

The high performance liquid chromatographic method was developed for the determination of chlorogenic acid in Flos Lonicerae. The chromato-graphic separation was carried out in a Nucleosil C18 column （4.6×250 mm, 5μm） with a mobile phase consisting of methanol-0.4 % H3PO4 （25：75, V/V） mixture.The detection wavelength of photodiode array detector was set at 327 nm and the column temperature was set at 25℃.The calibration curve was linear in the range of 0.64 - 6.40 μg for chlorogenic acid （r = 0.9996）. the average recovery of chlorogenic acid was 99.3 % with a relative standard deviation （RSD）of 0.8 % .the method was simple,reliable and suitable for quality evaluation and control of Flos Lonicerae.