人体鼻咽组织的时间分辨自体荧光光谱研究

Time-resolved Autofluorescence Spectroscopy for Classification of Human Nasopharyngeal Tissues

实验研究了在397 nm半导体脉冲激光激发下,人体离体鼻咽正常和癌变组织在600 nm荧光发射波长处的时间分辨自体荧光光谱特性。利用双指数衰减方程对时间分辨自体荧光光谱进行拟合后,获得相应的荧光强度随时间的指数衰减方程以及荧光平均寿命。人体鼻咽癌变和正常组织在600 nm处的自体荧光平均寿命分别为(2.94±0.51)ns和(4.29±0.71)ns,两者之间存在显著的差异。应用时间分辨光谱技术的诊断灵敏度和特异性分别为75%和100%。初步表明了时间分辨自体荧光光谱在早期鼻咽癌诊断的应用价值,该方法可望与传统的稳态荧光光谱结合起来,进一步提高早期鼻咽癌荧光诊断的准确率。

Time-resolved autofluorescence spectroscopies at 600 nm under 397 nm excitation have been measured for human nasopharyngeal normal and carcinoma tissues in vitro, respectively. The lifetimes of endogenous fluorophores can be indirectly calculated based on the two-component lifetime fitting of time-resolved autofluorescence spectroscopies. Sufficient difference was observed between normal and carcinoma tissues, and the average lifetimes of nasopharyngeal carcinoma and normal tissues are about （2.94±0.51 ） ns and （4.29 ±0.71 ） ns, respectively. In this prospective study, the sensitivity and specificity for diagnosis of nasopharyngeal carcinoma tissue are 75 % and 100 %. Experimental resuits indicate that time-resolved autofluorescence spectroscopy is a very sensitive and promising technique for classification of early nasopharyngeal carcinoma tissue.