不同浓度汞离子暴露下草鱼(Ctenopharyngodon idella)器官组织中酸性磷酸酶活性变化

Changes of Acid Phosphatase Activity in Different Organs and Tissues of Grass Carp(Ctenopharyngodon idella) Exposed to Hg^(2+)

草鱼(Ctenopharyngodon idella)暴露于汞离子不同浓度(0 mg.L-1、0.05 mg.L-1、0.10 mg.L-1、0.15 mg.L-10、.20 mg.L-10、.25 mg.L-1)下21 d,血清、鳃、肝胰脏、脾、肾和肌肉中酸性磷酸酶(Acid phospha-tase,ACP)活性显示,草鱼血清ACP活性在Hg2+0.15 mg.L-1下无显著变化;在汞离子>0.15 mg.L-1下对ACP活性有抑制作用.鳃中ACP活性随着汞离子浓度增加而降低.肝胰脏在汞离子浓度≥0.15 mg.L-1时,ACP活性显著升高(p<0.01).脾和肾中ACP活性随着汞离子浓度的增加呈现出先增加后降低的变化,低于0.15 mg.L-1时,随着浓度增加ACP活性上升;高于0.15 mg.L-1时,ACP活性则逐渐下降.肌肉ACP活性在汞离子0.15 mg.L-1下变化不显著(p>0.05);在汞离子>0.15 mg.L-1下酶活性显著下降(p<0.05).结论表明,草鱼暴露于不同浓度汞离子下,不同器官组织ACP活性呈现出不同的变化,主要是由于不同器官组织执行的生理功能不同,从而对汞离子胁迫表现出不同的应答反应,以提高机体的抗胁迫能力.

To study on metabolism enzyme change could be helpful for understanding the impacts of environmental factors on physiological regulation in fish. In this study, acid phosphatase （ACP） activities in serum, gill,hepatopancreas, spleen, kidney and muscle of Ctenopharyngodon idella exposed to different concentrations of Hg^2＋ ions for 3 weeks were measured. The results showed that ACP activity in serum decreased significantly at higher Hg^2＋ concentration （〉0.15mg·L^-1 ） （p〈0.01）. ACP activity in gill decreased gradually with the increase of Hg^2＋ concentration. ACP activity in liver significantly in- creased at ≥0.15 mg·L^-1 （p〈0.01）. ACP activity in spleen and kidney, with the rise of Hg2＋ concentration, increased grad- ually under 0.15 mg·L^-1 , and decreased gradually above 0.15mg·L^-1 , and compared to the control, there were significant differences at more than 0.10 mg·L^-1（p〈0.01）. ACP activity in muscle was significantly suppressive at only 0.20mg·L^-1 and 0.25mg·L^-1 （p〈0.01）. In conclusion, changes of ACP activities in different organs and tissues were contributed to maintaining its physiological functions and increasing the ability to be used to cope with the stress of Hg^2＋ ion.