利用盐溶蛋白PAGE电泳法检测玉米种子的研究

Study on the Method of Detecting the Maize Seeds by Salt-soluble Protein PAGE Electrophoresis

应用玉米盐溶蛋白PAGE电泳方法检测了10个玉米品种的纯度及其杂交种的真实性。通过品种间的盐溶蛋白谱带的对比,发现其中有2条蛋白带是各品种所共有的,而除这些共有的蛋白带以外,各品种还具有一些特异条带。根据每个品种特有的蛋白条带,建立其特征条带图谱,可应用于该品种的相关鉴定工作。

The purity and authenticity of maize seeds of 10 genotypes were detected with the method of the salt-soluble protein PAGE electrophoresis. With the contrast of the PAGE patterns of salt-soluble protein on these 10 varieties, we can find the common patterns of maize and the peculiar patterns of every special variety. Then the standard patterns of varieties can be established to identify a variety unknown.