胡萝卜种质资源遗传多样性的RAPD分析

Study on Germplasm Resources of Carrot(Daucus carota L.) Based on RAPD Analysis

利用RAPD技术对34份胡萝卜种质资源进行遗传多样性研究,以探明各材料间的亲缘关系、遗传多样性及其分类,为进一步利用和创新品种提供参考信息。结果表明,20条随机引物共扩增出139个DNA片段,其中99条带表现出多态性,占总带数的76.74%,对其进行数据化处理后聚类,34份胡萝卜材料可划分为5个类群。材料的归组与根形、根长有一定的相关性。

It is very important to detect the genetic diversity of carrot for understanding the origin, relationship and classifying of carrot and giving references to the development and utilization of carrot germplasm. Random amplified polymorphic DNA（RAPD） was used to analyze the genetic diversity of 34 carrot accessions in the present study. Twenty random primers was used to amplify the total accessions. Among the total 139 RAPD bands identified,99 bands（76.74% ） were polymorphic. Five groups were clustered by the digital data of polymorphic bands. There was a certain extent association between RAPD variation and morphological characteristics such as root-shape and mot-height of carrot.