食荚型豌豆组织培养和植株再生研究

Study on Tissue Culture and Plant Regeneration of Pea(Pisum sativum L)

以豌豆子叶、茎、真叶为材料进行外植体筛选及组织培养研究,试验结果表明:豌豆带节茎段是最佳试验材料;在MS附加1 mg/L BA和1 mg/L NAA培养基中,愈伤组织诱导率达100%,愈伤组织在分化培养基中未分化出芽;带节茎段在MS附加2～3 mg/L BA和0.1 mg/L NAA培养基中,腋芽和丛生芽产生率达100%,芽增殖系数3以上;不定芽在MS附加3 mg/L NAA上生根率86%;试管苗移栽成活率85%左右.

The cotyledon, stem and leaf of pea were cultured for selection of the appropriate explant. The result showed that stem with nod was the ideal material（better than other parts of the plant at callus induc tion）, the callus formation rate of stem with nod was 100% in MS with hormone BA 1 mg/L＋NAA 1 mg, L, and there were no buds in division culture. The productive rate of Axillary bud and Clump sprout reached 100% at the medium of MS＋BA 2-3 mg/L＋NAA 0. 1 mg/L, the quotiety of bud increment was above three and the rate of root growth was more than 86 % at the MS solid culture medium with NAA 2 mg/L. The surviving ratio of transplanted plant exceeded 85 %.