生长素对红叶石楠组培苗过氧化物酶和多酚氧化酶活性的影响

Effects on POD and PPO Activities during the Induction of Adventitious Root of Photinia frasery Treated with Different Auxins

试验对红叶石楠组培苗根诱导分化过程中过氧化物酶(POD)、多酚氧化酶(PPO)活性的变化进行了研究.结果表明,在使用IBA和NAA后,过氧化物酶活性在0～15 d先迅速下降,然后急剧上升,在根开始伸长时,活性又开始下降;多酚氧化酶活性在0～15 d内逐步上升,在根开始伸长时,活性也开始下降.而在不使用任何生长调节剂的情况下则不生根,过氧化物酶活性在0～15 d内逐步上升,15 d后开始下降;多酚氧化酶活性在整个根诱导过程中都维持在较低水平.同时还发现,在生根过程中,处理与对照相比,各酶的活性差异达极显著水平,表明红叶石楠组培苗的不定根发生和发育与过氧化物酶、多酚氧化酶的活性密切相关.

The activities of peroxidase and polyphenol oxidase during the induction of adventitious root of Photinia frasery were investigated. The results showed that the peroxidase activity during the development of adventitious root decreased and then increased rapidly in 0-15 days; when roots began elongation, the decrease of peroxidase activity was observed. The polyphenol oxidase activity in adventitious root development increased in 0-15 days, then decreased after roots began elongation. Meanwhile, the peroxidase activity during the development of the adventitious root in 0-15 days increased and then decreased at no any auxin treatment, while the polyphenol oxidase activity was very low during the whole development of the adventitious root. Compared with the control, the differences of both enzymes activities in rooting were very significant. Therefore, it indicated that the induction and development of the adventitious roots in Photinia frasery were relative to the activities of peroxidase and polyphenol oxidase.