应用大孔吸附树脂分离纯化葶苈子总黄酮的研究

Separation and purification of total flavones from Semen Descuraniae by macroporous adsorption resin

目的考察6种大孔树脂对葶苈子总黄酮成分的分离纯化性能,确定最佳工艺条件。方法以总黄酮吸附量、洗脱率、洗脱物总黄酮含量为指标,采用紫外-可见分光光度法进行含量测定。结果NKA-Ⅱ型树脂对葶苈子总黄酮具有较好的纯化分离能力,其最佳工艺条件为:葶苈子提取物浓度4g/mL(生药量),最大吸附量12.92mg/g,装柱径高比1∶12,吸附流速3mL/min,洗脱剂80%乙醇,洗脱量5倍柱体积,洗脱流速3mL/min。结论NKA-Ⅱ型树脂纯化分离葶苈子总黄酮的最佳工艺稳定高效,可推广用于生产。

Objective To establish the purification and separation technology for total flavones from Semen Descuraniae by investigating 6 types of macroporous adsorption resin. Method The adsorption capacity, elution ratio and purify of total flavones were used as evaluating criteria. The UV-VIS spectrophotometric method was used in the determination of total flavones content. Results NKA-11 resin had better property of purification and separation. The optimal technological conditions were： the concentration of the sample of Semen Descuraniae, 4 g/ml （amount of crude drug ）; the maximum adsorption capacity for total flavones, 12.92 mg/g ; the optimal column ratio of diameter to height, 1/12 ; the flow velocity of adsorption,3mL/min ; the eluting reagent, 80% ethanol（5 times of the volume of the resin） ;eluting velocity , 3mE/min. Conclusion The technology of separating and purifying total flavones from Semen Descuraniae with NKA- 11 was stable and effective, and can be applied to manufacture.