共轭亚油酸对肉鸡免疫指标的影响

Effect of Conjugated Linoleic Acids on Immune Function of Broilers

选择1d艾维因肉鸡360羽，按单因素设计随机分为5个处理，每处理设3个重复，每重复24只鸡，分别饲喂添加0％、0．5%，5、1．0％、1．5%和2．0％CLA的试验日粮，旨在探讨CLA添加水平对内鸡免疫功能的影响及相关机理。结果表明，CLA添加显著影响0-35d肉鸡的免疫功能，但对36-49d影响并不明显。21d和35d0．5%添加组胸腺指数最高，分别为4．07g／kg和2．68g／kg，显著高于相应阶段对照组（P〈0．05），而脾脏指数和法氏囊指数却以1．0％添加组较高，并显著高于对照组（P〈0．05）。T-淋巴细胞百分率和抗体滴度测定结果也表明，在肉鸡生长的不同阶段，ANAE＋（％）和ND抗体滴度均以1．0% ACLA添加组最高，21d和35d明显高于对照组、1．59，6和2．0%添加组（P〈0．05），但与0．5%添加组差异不显著（P〉0．05）。提示CLA添加对内鸡免疫功能的影响可能存在剂量效应和时间效应。

360 avain broilers one day old were selected and allocated to 5 treatments, each treatment has 3 replicates of 24 broilers at random, to investigate the effect of CLA supplement level on immune function of broilers and relative mechanisms. The experiment al diet was supplemented with 0%, 0.5%, 1.0%, 1.5% and 2.0% CLA respectively. Results showed that CLA supplementation had obvious effect on broilers＇ immune function at 0-35d, while had no effect at 36-49d. At 21d and 35d,the highest thymus index was found in 0. 5% CLA supplementation group, they are 4. 07 g/kg and 2.68 g/kg, which was higher than the control （ P 〈 0.05 ） and other CLA additive groups at corresponding stage. While the highest spleen index and fabric bursa index were found in 1.0 % group, which were higher than control （ P 〈 0.05 ）and other CLA additive groups at corresponding stage. The results of T-lymphocyte（ANAE＋%）and Newcastle Disease（ND ）ticter analyze showed that ANAE＋（%） and ND titer of 1.0% group was the highest at different growth stage in broilers, which were significantly higher than the control group, 1.5% and 2. 0% CLA supplement group（ P 〈 0.05 ） at 21d and 35d,but there is no difference between 0.5% supplement group（ P 〉 0.05 ）. It indicated that the influence of CLA supplementation on immune function in broilers could exist a dose and time effect.