高频饮用牛奶和葡萄糖对雌性大鼠生殖机能的影响

Influence of Drinking Milk and Sugar Frequently on the Reproductive Performance of Female Rats

选用22d断奶SD大鼠高频饮用牛奶或葡萄糖40d,分别记录各组动物的阴道开张时间和阴道黏液、每周日增重、卵巢及子宫所占体重百分比、血糖水平,收集卵巢进行组织学处理以观察卵泡发育情况,探讨高频饮用牛奶和葡萄糖对雌性大鼠初情期的影响。结果显示,奶组和糖组大鼠较对照组阴道开张时间来得早,预示初情期可能提前,而且,奶组和糖组大鼠的卵巢占体重百分比较对照组高(P<0.05)。牛奶组血糖水平比对照组高,但糖组与对照组血糖水平差异不显著。通过对卵巢进行石蜡切片、HE染色及卵泡计数,结果显示:奶组的闭锁卵泡、每张切片卵泡总平均数都比对照组多(P<0.05),而葡萄糖组的黄体数比对照组少(P<0.05)。试验表明高频饮用牛奶和葡萄糖可对性成熟前雌性大鼠的生殖机能产生一定影响。

The current experiment examined the effects of milk and sugar drunk frequently on pUberty in immature female rats. The 22 daysold weaned rats drank milk and sugar daily instead of tip water for 40 days. The experimental animals were examined daily with the vaginal open day and checked smear everyday afterwards, weighted body weight every week during experimental period. In the end of experiment, weights of the ovary and uterus, levels of blood sugar and morphometric of ovaries were examined. The results showed that the vaginal open day of treated group is earlier than that of the control group, which might imply that the puberty arrived earlier by drinking milk and sugar. In addition, the ratio of ovarian weight to body weight was increased with milk and sugar drinking. Furthermore, numbers of developmental follicles, atretic follicles and corpora luteal were increased in the treatment of milk compared with the control group, while in sugar group, only the number of corpora luteal was increased compared with the control group. Together, the results of the current experiment suggested that drinking milk and/or sugar has potential effects on puberty of weaned rats.