摘要
研究了不同品种间愈伤组织诱导及植株再生的差异.结果表明,MS+2,4-D(2.0 mg/L)+6-BA(0.5mg/L)+蔗糖0.3%+琼脂0.08%,MS+KT(0.5 mg/L)+NAA(0.01 mg/L)+蔗糖0.3%+琼脂0.08%,1/2MS+NAA(0.01 mg/L)+蔗糖0.15%+琼脂0.08%分别是紫花苜蓿的愈伤组织诱导、芽、根分化的适宜诱导培养基.
This paper is to probe into the difference of callus induction and plants regeneration among different alfalfa cultivars. The results showed that the most suitable for restoring the tissue,bud and root were as follows : MS+ 2,4-- D(2.0 rag/L) + 6-- BA (0.5 rag/L) + sucrose 0.3 % + agar 0.08 %, MS + KT (0.5 mg/L)+NAA(0.01 mg/L)+sucrose 0.3%+agar 0.08%,1/2MS+NAA(0.01 mg/L)+sugar 0.15 % +agar 0.08 % separately.
出处
《甘肃农业大学学报》
CAS
CSCD
2007年第1期77-81,共5页
Journal of Gansu Agricultural University
基金
国家自然科学基金项目(30371745)资助
关键词
紫花苜蓿
子叶节
下胚轴
愈伤组织
植株再生
Medicago sativa L.
hypocotyl
hypocotyls
callus
plant regeneration