秋水仙碱诱导番茄多倍体的研究

Studies on Polyploid Induction of Tomato with Colchicine

以巨宝金冠和毛粉802的种子和幼苗为试材,以秋水仙碱为诱变剂诱导多倍体。结果表明:处理番茄种子,以秋水仙碱浓度0.4%处理12 h效果最好,巨宝金冠和毛粉802的诱导率分别为26.7%和30%;处理番茄幼苗,以秋水仙碱浓度为0.03%处理8 h效果最好,巨宝金冠和毛粉802的诱导率分别为46.7%和50%。变异材料的细胞染色体(2n=4x=48)为2倍体材料的二倍。此外,四倍体变异材料均表现出叶长、叶宽、叶面积增大,叶形指数减少等特征。

Seeds and seedlings of Jubaojinguan and Maofen802 were treated with colchicines. The results indicated that the highest induction rate of Jubaojinguan and Maofen802 were 26.7%and 30% with optimum treatment combination of 0. 4%/12h and treating the seeds,46.7% and 50% with optimum treatment combination of 0. 03%/8h and treating the seedlings. The chromosome number of the mutated tomato （2n=4x=48） was twice that of the control. In addition, the leaf length, width and are a were larger ahd the leaf index was smaller than those of the diploid plants.