组织蛋白酶B在化疗药物Etoposide作用后肝癌细胞中表达的研究

Surgical treatment in the dumbbell tumors of the craniocervical junction and cervical spinal canal

目的初步探讨组织蛋白酶B(CB)在化疗药物Etoposide作用后肝癌细胞中的表达及其意义。方法MTT法检测不同浓度(0~600μg/ml)Etoposide作用24h后HepG2细胞存活率,琼脂糖电泳法检测细胞凋亡DNA片段化,荧光定量PCR检测CB表达水平。结果不同浓度(2.4~600μg/ml)Etoposide作用HepG2细胞24h后细胞存活率显著下降,且与药物浓度呈负相关(R=-0.81,P=0.00);琼脂糖电泳法检测细胞凋亡DNA片段化未见典型梯状条带,CB表达水平增加(2.8±0.43)^(3.91±0.02)倍,与对照组比较差异有统计学意义(P=0.00),并与细胞存活率呈负相关(r=-0.94,P=0.00)。结论CB在Etoposide作用后HepG2细胞表达增高,提示药物治疗后有可能通过激活CB表达而促进残留肿瘤细胞的侵袭和转移。

Objective To explore approaches to remove dumbbell tumors of the craniocervical junction and cervical spinal canal, Methods The modified far lateral suboccipital approach and the post-middle approach were used in 26 patients with dumbbell tumors of the craniocervical junction and cervical spinal canal,the clinical data ,were analyzed retrospectively, Results There were total removal tumor in 23 cases at one-stage operation,and subtotal removal in 3 cases. There was no operative mortality. No complications occurred due to the operation, Postoperative follow-up ranged flora 1 to 9 years, all patients got good recovery,except one subtotal resected chordoblastoma relapsed in two years. Conclusion The modified far lateral suboccipital and the post-middle approach are effective to remove the dumbbell tumors of the craniocervical junction and cervical spinal canal,which are characterized by wide operation field,good tumor exposure,and high total removal ratio.