金黄色葡萄球菌氟喹诺酮的耐药性与grlA、grlB突变的关系

Relation between fluoroquinolones-resistant and mutation of grlA and grlB gene in Staphylococcus aureus

为了探讨金黄色葡萄球菌的氟喹诺酮耐药性与grlA、grlB基因突变的关系,从52株野生型金黄色葡萄球菌中筛选出1株对氟喹诺酮敏感的金黄色葡萄球菌,对其进行耐药诱导,获得一系列不同氟喹诺酮耐药水平的金黄色葡萄球菌,并对其grlA、grlB基因进行PCR扩增、测序及序列分析。结果表明,金黄色葡萄球菌的氟喹诺酮耐药性与grlB基因突变无关,而与grlA的丝氨酸(Ser)80→苯丙氨酸(Phe)和谷氨酸(Glu)84→赖氨酸(Lys)突变密切相关。金黄色葡萄球菌grlA的80位氨基酸和84位氨基酸双突变是其氟喹诺酮耐药性提高的标志之一。

The purpose was to study the relation between fluoroquinolones-resistant and mutation of grlA and grlB gene in staphylococcus, aureus,the sensitive strain was induced with Ciprofloxacin to get grad-resistance strain of S, aureus, the grlA and grlB gene were amplified and sequenced by polymerase chain reaction. Sequence analysis showed that grlB gene had no mutation,while Ser 80→Phe and Glu 84→ Lys of the grlA gene happened. These assays demonstrated that Ser 80→Phe and Glu 84→Lys of the grlA mutation were the main mark of staphylococcus aureus to {luoroquinolones-resistant rising.