酸枣仁颗粒中酸枣仁皂苷A和酸枣仁皂苷B的含量测定

Determination of Jujubaside A and Jujubaside B in Ziziphus Jujuba Mill Granules

目的建立酸枣仁颗粒中酸枣仁皂苷A和酸枣仁皂苷B的含量测定方法。方法采用反相高效液相色谱法,分析柱为Hypersil C18柱,以乙腈和水为流动相的二元梯度洗脱方法,检测波长为201nm,流速为1mL/min。结果酸枣仁皂苷A进样量在0.50～1.98μg与峰面积呈良好的线性关系(r=0.9998,n=5),平均回收率为99.41%;酸枣仁皂苷B进样量在0.66～2.09μg与峰面积呈良好的线性关系(r=0.9998,n=5),平均回收率为99.34%。结论该法简便、快速、重现性好,适用于酸枣仁颗粒中酸枣仁皂苷A、酸枣仁皂苷B的定量分析。

Objective To develop a method for the determination of jujubaside A and jujubaside B in ziziphus jujuba mill granules （ZJMG）. Methods A RP- HPLC method was set up, using Hypersil 18 column. The mobile phase consisted of acetonitrile and water, and a gradient elution program was applied. The DAD detector was set at 201 nm. Results The calibration curve of jujubaside A was linear in the range of 0.50-1.98 μg. The average recovery rate was 99.41%.The calibration curve of jujubaside B was linear in the range of 0.66-2.09 μg. The average recovery rate was 99.34%. Conclusion The method is appropriate for the determination of jujubaside A and jujubaside B.