纳米化几丁聚糖对诱变剂引起的沙门菌回复突变的拮抗作用

Antagonism of Nano-chitosan against Mutagenic Effect of Different Mutagens to Salmonella Typhinurium

目的研究纳米化几丁聚糖对诱变剂引起的鼠伤寒沙门菌回复突变的拮抗作用。方法采用L12(3×44)正交设计,使几丁聚糖4个剂量组与9-氨基吖啶(9-AA)、2,7-氨基芴(2,7-AF)、叠氮纳(NaN3)、丝裂霉素C(MMC)、2-乙酰氨基芴(2-AAF)各3个剂量组均能交互作用于试验菌株各1次。通过平板掺入法进行Ames试验,每株菌+/-S9体外活化系统各设3个平行皿,计数每皿回变菌落数。结果加或不加S9体外活化系统时,在实验剂量范围内(纳米化几丁聚糖剂量为0、20、50、100mg/皿,诱变剂9-AA、2,7-AF、NaN3和2-AAF剂量为1、5、10μg/皿,MMC剂量为1、2、3μg/皿),纳米化几丁聚糖可使诱变剂诱导的TA97、TA98、TA100和TA102菌株回复突变率均降低到阳性标准之下。结论纳米化几丁聚糖可以降低不同诱变剂引起的相应的鼠伤寒沙门菌株的回复突变,并呈现剂量依赖性。

[ Objective ] To study the antagonism of nano - chitosan against mutagenic effect of different mutagens to salmonella typhinurium. [ Methods] LI2 （ 3×4^4） orthogonal design was employed to ensure that the 4 doses of nano -chitosan could interact with the doses of 9AA, 2, 7AF, NAN3, MMC and 2 -AAF respectively; Ames test was performed by the plate incorporation method, 3 parallel plates were used with or without the S9 system and the colonies were counted. [ Results ] With or without the S9 system, nano- ehitosan could reduce the colony number to be below the positive criteria within the experimental dose range （ the doses of nano - chitosan were 0, 20, 50 and 100 mg/plate; the doses of mutagenic agent 9AA, 2,7AF, NaN3 and 2AAF were 1,5 and 10 μg/plate; the doses of MMC were 1,2 and 3 μg/plate）. [ Conclusion] Nano - chitosan could reduce the reverse mutation of salmonella typhinuriura induced by different mutagenic agents with dose dependence.