毛细管电泳电导法快速测定西米替叮的方法

Study on Determination of Cimetidine by High Performance Capillary Electrophoresis with Conductivity Detection

建立了毛细管电泳电导法快速测定西米替叮的方法.利用非涂层弹性融硅石英毛细管(50cm×75μm)为分离通道,探讨了缓冲溶液的种类、pH值、浓度,分离电压,进样时间对分离检测的影响.结果表明,在最佳条件下:30mmol/LTris(Cit调节pH=8.0)为运行缓冲介质,分离电压为10kv,西米替叮在10分钟内能得到较好的检测.标准品溶液浓度在0.5-20mg/L范围内有良好的线性关系,回归方程为y=9.0923x+15.83,相关系数r=0.9984,回收率为98.8%.

A quick and simple method of high performance capillary electrophoresis with conductivity detection was established for the detection of Cimetidine. Separation was carried out in a uncoated fused silica capillary （50 cm×75 μm）. Effects of several important factors were investigated such as the pH and concen- tration of running buffer, operation voltage, injection time, injection height. Cimetidine can be detected in nine minutes and forty seconds under the optimized condition： 30mmol/LTris ＋ 100 mmol/Lcit （pH ：8.26） as running buffer, 10kv operation voltage. The concentration of Cimetidine was lined with＇the peak height in the range of0.5~20 mg/L, h：9.0923c ＋ 15.83,r=0.9984.Recovery rate was 98.8%.