加味玉屏风散对哮喘大鼠支气管肺泡灌洗液中细胞因子水平的影响

Study on change and correlation of cytokine in broncho-alveolar lavage fluid in asthmatic rat and the effect of augmented jade screen powder (加味玉屏风散) on their contents

目的:探讨白细胞介素-5(IL-5)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、肿瘤坏死因子-α(TNF-α)和可溶性白细胞介素-2受体(sIL-2R)在哮喘发病过程中的作用及补脾益气中药加味玉屏风散对其含量的影响。方法:将24只大鼠随机分为正常对照组、哮喘模型组和加味玉屏风散组,每组8只。采用卵白蛋白、灭活百日咳杆菌疫苗和氢氧化铝干粉配制的溶液腹腔注射致敏以及卵白蛋白雾化吸入激发制备大鼠哮喘模型;正常对照组用等量生理盐水替代抗原液;加味玉屏风散组则胃饲加味玉屏散水溶液。观察支气管肺泡灌洗液(BALF)中细胞总数及细胞分类,以及IL-5、GM-CSF、TNF-α和sIL-2R含量的变化。结果:与正常对照组比较,哮喘模型组BALF中IL-5、GM-CSF、TNF-α和sIL-2R含量均显著增高(P<0.05或P<0.01),尤其是IL-5增高更加显著;相关分析表明,GM-CSF与IL-5、GM-CSF与TNF-α均呈显著正相关(r1=0.519,r2=0.521,P均<0.05)。加味玉屏风散能降低BALF中这4种细胞因子的含量(P<0.05或P<0.01)。结论:IL-5、GM-CSF、TNF-α和sIL-2R在哮喘大鼠的气道炎性细胞浸润过程中起重要作用,可能加速了哮喘非特异性气道炎症的发生发展,并对嗜酸粒细胞的聚集、迁移、活化和黏附有协同作用。加味玉屏风散可以通过降低细胞因子水平来改善气道的炎症状况。

Objective： To discuss the actions of interleukin - 5 （IL - 5）, granulocyte - macrophage colony - stimulating factor （GM - CSF）, tumor necrosis factor -α （TNF -α） and soluble interleukin - 2 receptor （sIL - 2R） in the course of asthma attack and the effect of spleen - Qi nourishing Chinese herbs [补脾益气中药, augmented jade screen powder （加味玉屏风散）] on these contents. Methods： Twenty- four male rats were randomly divided into three groups： normal control group, asthmatic model group and augmented jade screen powder group （each n = 8）. A sensitized asthmatic rat model was obtained by intraperitoneal injection of egg albumin, inactivated Bordetella pertussis vaccine and a solution prepared from aluminum hydroxide dry powder, and provocation by breathing in egg albumin via an atomizer. Normal control group was supplied with equal amount of physiological saline to substitute antigen liquid; augmented jade screen powder group was fed with augmented jade screen powder solution. Total cellular score and cellular classification in broncho -αlveolar lavage fluid （BALF） were observed, and the same with the changes of the contents of IL - 5, GM - CSF, TNF -α and sIL - 2R. Results ： Compared with the normal control group, the BALF in the asthmatic model group had a dramatic increase of the contents of IL - 5, GM -CSF, TNF -α and sIL -2R （P〈0.05 or P〈0.01） , especially the IL- 5, the correlation analysis showed that GM -CSF and IL-5, GM -CSF and TNF-α were significantly positively correlated （r1 = 0.519, r2 = 0.521, both P〈0.05）. Augmented jade screen powder could decrease the contents of these four types of cellular factors in BALF （P〈0.05 or P〈0.01） . Conclusion： IL - 5, GM -CSF, TNF -α and sIL - 2R are of great importance during the course of infiltration of the airway inflammatory cells in the asthmatic rat. They may accelerate the emergence and development of the nonspecific airway inflammation of asthma. It is expected that this has synergistic action to the aggregation, migration, activation and adherence of the eosinophil （EOS）. Augmented jade screen powder can improve the airway＇s inflammation state through depressing the levels of various cellular factors.