益气活血解毒汤对实验性动脉粥样硬化家兔炎症细胞因子和血脂的影响

Effects of Yiqihuoxuejiedu decoction (益气活血解毒汤) on C-reactive protein,interleukin-6,tumor necrosis factor and serum lipid in experimental rabbits with atherosclerosis

目的:观察中药复方益气活血解毒汤对实验性动脉粥样硬化(AS)家兔血脂及炎症细胞因子水平的影响,探讨该方防治AS的机制。方法:将24只日本雄性大耳白兔随机分为正常对照组、模型组、益气活血解毒汤组和辛伐他汀组,每组6只。采用高脂饲料喂饲建立兔AS模型,其中益气活血解毒汤组及辛伐他汀组在制模的同时分别给药,而正常对照组和模型组以等量蒸馏水灌胃。10周后测定各组空腹血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、C-反应蛋白(CRP)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)含量。结果:模型组TG、TC、LDL-C、CRP、IL-6及TNF-α均较正常对照组有不同程度的升高,HDL-C明显下降,差异均有显著性(P均<0.01)。益气活血解毒汤组及辛伐他汀组TG、TC、LDL-C、CRP、IL-6及TNF-α均明显低于模型组,HDL-C则明显升高,差异均有显著性(P均<0.01),而与正常对照组比较差异无显著性(P均>0.05)。益气活血解毒汤组HDL-C较辛伐他汀组有所升高,差异有显著性(P<0.05)。结论:益气活血解毒汤对AS有明显的预防和治疗作用;调节血脂和降低炎症细胞因子水平可能是其作用机制之一。

Objective： To observe the influence of Yiqihuoxuejiedu decoction （益气活血解毒汤） on levels of serum lipid, C- reactive protein （CRP）, interleukin- 6 （IL -6） and tumor necrosis factor -α （TNF-α） in rabbits with atherosclerosis （AS） and investigate its mechanism. Methods： Twenty- four Japanese white rabbits with large ears were randomly divided into four groups： normal control group, model group, Yiqihuoxuejiedu decoction group and simvastain group （each n = 6）. The model of AS was established by feeding the fodder containing cholesterol ,the drugs were administered along with the replication of the model in Yiqihuoxuejiedu decoction group and simvastain group, and equal quantity of distilled water was administered via an intragastric route in the former two groups. After 10 weeks, the concentrations of triglyceride （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （HDL - C）, CRP, IL - 6 and TNF -α of each group were measured. Results ： Compared with the normal control group, the levels of TG, TC, HDL -C ,CRP,IL -6 and TNF -α of model group increased in various degrees, while HDL -C decreased, the differences being significant （all P〈0.01）. Compared with the model group, the concentrations of TG, TC, LDL - C, CRP, IL - 6 and TNF -α of Yiqihuoxuejiedu decoction group and simvastain group decreased obviously, while HDL -C increased obviously, the differences being significant （all P〈0.01）. But the results of the above two groups had no significant differences in comparison with those of the control group （all P〉0.05）. Compared with the simvastain group, there was statistical significant difference of the increased level of HDL - C in Yiqihuoxuejiedu decoction group （P 〈0.05）. Conclusion： Yiqihuoxuejiedu decoction is a potential drug in treating and preventing atherosclerosis. Probably one of the mechanisms of Yiqihuoxuejiedu decoction depends mostly on regulating the serum lipid and decreasing the inflammatory factors, such as CRP,IL- 6 and TNF-α.