木聚糖酶产生菌黑曲霉SP-1液体发酵条件的研究

Study on Fermentation Condition with Xylanase Producing of Aspergillus Niger SP-1

研究了碳源、氮源以及其他因子对产木聚糖酶高产菌株黑曲霉（Aspergillus niger）SP-1产酶的影响，结果表明：当碳源为70g／L玉米芯，氮源3g／LNaNO，和3g／L（NH4）2SO4，pH值在5．4—6．0时，黑曲霉SP-1的酶活超过300IU／mL。采用玉米芯代替半纤维素作为碳源可以大大的降低成本，减少环境污染，有利于木聚糖酶的工业化生产。

Effects of carbon source,nitrogen souce and other factors on xylanase production of Aspergillus niger SP-1 were studied.The results showed the optimal conditions for enzyme formation were 70 g/L corn-cob,3 g/L NaNO3 and 3g/L(NH4)2SO4,pH5.4～6.0.The xylanase acitivity was above 300 IU/mL.