期刊文献+

高效液相色谱法测定消炎止咳片中穿心莲内酯和脱水穿心莲内酯的含量 被引量:1

HPLC Determination of Andrographolide and Dehydroandrographolide in Xiaoyan Zhike Tablets
下载PDF
导出
摘要 目的建立高效液相色谱(HPLC)法测定消炎止咳片中穿心莲内酯和脱水穿心莲内酯含量。方法采用(Agilent)ZORBAX XDB C18(4.6 mm×150 mm,5μm)色谱柱,检测波长250nm,流动相:甲醇-水(55:45)流速:1.0 mL.min-1柱温:25℃。结果穿心莲内酯线性范围为14.5~145.0μg.mL-1(r=0.999 8);脱水穿心莲内酯线性范围为11.7~117.0μg.mL-1(r=0.999 9),穿心莲内酯平均回收率为98.8%,脱水穿心莲内酯平均回收率为99.4%。结论该方法分离效果好、精密度高、准确性好,可以作为消炎止咳片中穿心莲内酯和脱水穿心莲内酯含量测定的方法。 Objective To establish an HPLC determination of andrographolide and dehydroandrographolide contents in xiaoyanzhike tablets. Methods A ZORBAX XDB-C18 Column (4. 6 mm×150 mm, 5 μm) was used, methanol -water (55 : 45) was used as mobile phase , the detection wavelength was 250 nm, flow rate 1.0 mL·min^-1 and column temperature at 25 ℃. Results Linear rang of andrographolide was 14. 5 - 145. 0 μg ·mL^-1 (r = 0. 999 8 ), linear rang of dehydroandrographolide was 11.7 - 117.0 mg· mL^-1 ( r = 0.999 9) , the average recovery of andrographolide was 98.8 % and the average recovery of dehydroandrographolide was 99.4 %. Conclusion The method is simple, accurate , reliable and can be used to control the quality of this preparation.
出处 《医药导报》 CAS 2007年第9期1079-1080,共2页 Herald of Medicine
关键词 消炎止咳片 穿心莲内酯 脱水穿心莲内酯 色谱法 高压液相 Xiaoyan zhike tablets Andrographolide Dehydroandrographolide HPLC
  • 相关文献

参考文献2

二级参考文献3

共引文献26

同被引文献4

引证文献1

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部