^(12)C^(6+)重离子辐照大葱的生物学效应

Biological effects of ^(12)C^(6+) heavy ions irradiation on Allium fistulosum L.

用30Gy、90Gy和180Gy 12C6+重离子辐照处理大葱干种子,研究了它对大葱的生物学效应。经过不同剂量12C6+重离子照射过的大葱,幼苗发芽率、株高等表型随着辐照剂量的增大,呈现明显的"抛物线"趋势。适量的12C6+重离子照射(30Gy)能提高大葱发芽率和抗旱、抗倒伏能力,促进生长发育。重离子辐照能有效地诱导大葱根尖细胞微核和染色体畸变形成,180Gy处理的大葱微核率和染色体畸变率最高达到9.09%和10.03%。本实验为大葱的重离子辐照育种打下基础。

In order to study biological effects of ^12C^6＋ heavy ions irradiation on Allium fistulosum L., the dry seeds were treated by ^12C^6＋ heavy ion beam with different doses. Obvious ＂parabola＂ trends were observed in the characteristics of germination rate and height ofAlliumfistulosum L. seedling when irradiated doses increased. With irradiation of an appropriate dose of ^12C^6＋ heavy ion beam, 30Gy, the germination rate ofAlliumfistulosum L. is increased and the seedlings can resist drought and lodging, and grow better than the control. At the same time, the formarion frequency of micronucleus and chromosomal aberration were surveyed in mot-tip cells. The highest formation frequency of micronucleus and chromosomal aberration treated with 180Gy could reach to 9.09% and 10.03% respectively. This study laid the basis for further work on breeding and improvement of Allium fistulosum L. irradiated by ^12C^6＋ heavy ion beam.